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This procedure allows the screening of a high number of single transformant colonies (E.coli) for the presence (and/or the length) of a specific DNA sequence. In order to get reliable results, it is advisable to set up qPCR reactions starting from O/N cultures rather than from E.coli colonies. Briefly, selected colonies from a plate should be inoculated in 1-5 mL of LB and grown O/N. O/N cultures will be assayed and t...