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Created on: 25-Nov-2013. Updated on: 27-May-2015.

Category: Bacteria.

Preparation of Competent Cells - Walker Protocol

Posted by: dami82, UIC, Chicago, IL, http://www.biotechworld.it/portfolio/fantini


IMPORTANT: everything should be kept on ice during the experiment
  1. Start an O/N culture of E.coli in 2-5 mL of LB Broth (use the appropriate antibiotic if needed, i.e. Chloramphenicol for RosettaTM strains)
  2. Inoculate 200 mL LB with 3ml of O/N E.coli suspension
  3. Grow at 37° in shaker (about 200rpm) for about 1-1.5h (culture has to be in exponential growth: wai...

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